Novel method to investigate thermal exchange rates in small, terrestrial ectotherms: A proof-of-concept on the gecko Tarentola mauritanica

by Gabriel Mochales-Riaño, Frederico M. Barroso, Valéria Marques, Alexandra E. Telea, Marco Sannolo, Catarina Rato, Miguel A. Carretero

Thermoregulating ectotherms may resort to different external heat sources to modulate their body temperature through an array of behavioural and physiological adaptations which modulate heat exchange with the environment and its distribution across the animal’s body. Even small-bodied animals are capable of fine control over such rates and the subsequent re-allocation of heat across the body. Such thermal exchanges with the environment usually happen through two non-mutually exclusive modes: heliothermy (radiant heat gain from the sun) or thigmothermy (heat gained or lost via conduction). Classically, the study of these phenomena has relied on invasive methodologies which often disregard the effect of stress, behaviour and regional heterothermy on the rates and patterns of thermal exchange across the body of the animal. This study proposes a novel experimental methodology, capitalising on thermography, to provide an alternative method to less invasively obtain reliable body temperatures of thermoregulating ectotherms, while allowing behaviour and heating mode to be considered when quantifying thermal exchange rates. This methodology was tested in the gecko Tarentola mauritanica, where twenty males were allowed to heat up and cool down under a novel experimental set-up which isolates heliothermic and thigmothermic processes, while being recorded with a thermal camera. The study revealed differences in the heating and cooling rates of several body parts per treatment suggesting that thermal exchanges are complex even in small ectotherms. Ultimately, the described set-up provides the opportunity to revisit classical questions with a less invasive and more flexible experimental approach, enabling heliothermic and thigmothermic processes to be disentangled. The described methodology also better integrates behaviour and physiology while obtaining higher temporal and spatial resolution of body temperatures in a thermoregulating ectotherm.