Overexpression of TCP9-like gene enhances salt tolerance in transgenic soybean

by Zhuo Zhang, Yuanling Zhao, Yifan Chen, Yueming Li, Lijun Pan, Siyu Wang, Piwu Wang, Sujie Fan

TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors are a plant-specific family and play roles in plant growth, development, and responses to biotic and abiotic stresses. However, little is known about the functions of the TCP transcription factors in the soybean cultivars with tolerance to salt stress. In this study, TCP9-like, a TCP transcription factor, was identified in the soybean cultivars exposed to salt stress. The expression of TCP9-like gene in the roots of salt-tolerant soybean cultivars was higher than that in salt-sensitive cultivars treated with NaCl. The overexpression of TCP9-like enhanced the salt tolerance of the salt-sensitive soybean cultivar ‘DN50’. In T2 generation, the plants with TCP9-like overexpression had significantly lower Na⁺ accumulation and higher K⁺ accumulation than the WT plants exposed to 200 or 250 mmol/L NaCl. The K⁺/Na⁺ ratio in the plants overexpressing TCP9-like was significantly higher than that in WT plants treated with 200 mmol/L NaCl. Meanwhile, the overexpression of TCP9-like up-regulated the expression levels of GmNHX1, GmNHX3, GmSOS1, GmSOS2-like, and GmHKT1, which were involved in the K⁺/Na⁺ homeostasis pathway. The findings indicated that TCP9-like mediated the regulation of both Na⁺ and K⁺ accumulation to improve the tolerance of soybean to salt stress.