Sequence and epigenetic landscapes of active and silent nucleolus organizer regions in Arabidopsis | Science Advances
Abstract
Arabidopsis thaliana
has two ribosomal RNA (rRNA) gene loci, nucleolus organizer regions
NOR2
and
NOR4
, whose complete sequences are missing in current genome assemblies. Ultralong DNA sequences assembled using an unconventional approach yielded ~5.5- and 3.9-Mbp sequences for
NOR2
and
NOR4
in the reference strain, Col-0. The distinct rRNA gene subtype compositions of the NORs enabled the positional mapping of their active and inactive regions, using RNA sequencing to identify subtype-specific transcripts and DNA sequencing to identify subtypes associated with flow-sorted nucleoli. Comparisons of wild-type and silencing-defective plants revealed that most rRNA gene activity occurs in the central region of
NOR4
, whereas most, but not all, genes of
NOR2
are epigenetically silenced. Intervals of low CG and CHG methylation overlap regions where gene activity and gene subtype homogenization are high. Collectively, the data reveal the genetic and epigenetic landscapes underlying nucleolar dominance (differential NOR activity) and implicate transcription as a driver of rRNA gene concerted evolution.